Stopped-Flow

Stopped-flow measurements involve the rapid mixing of two or more solutions and observation of the resulting kinetic data. Utilizing a Stopped-flow system with the J-815 allows measurement of Stopped-flow CD, Stopped-flow Absorbance and Stopped-flow Fluorescence all on the same instrument.

JASCO has teamed with Bio-Logic, the world leader in high end Stopped-flow instrumentation to couple their SFM series to the J-815 to allow seamless control, data acquisition and analysis. Two, three and four syringe models are available offering flexible mixing as well as upgradeable to quench-flow and T-jump capability. Stepper-motor driven syringes allow infinitely variable mixing ratios and a mechanical mixer efficiently mixes solutions of different viscosities commonly used in protein folding experiments. Seamless control of both the CD spectrometer and stopped-flow system is accomplished from a single window.

 

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