Stopped-Flow

Stopped-flow measurements involve the rapid mixing of two or more solutions and observation of the resulting kinetic data. Utilizing a Stopped-flow system with the J-815 allows measurement of Stopped-flow CD, Stopped-flow Absorbance and Stopped-flow Fluorescence all on the same instrument.

JASCO couples the new SFS-490 Series stopped-flow to the J-815 to allow seamless control, data acquisition and analysis. Two, three and four syringe models are available offering flexible mixing as well as upgradeable to T-jump capability. Stepper-motor driven syringes allow infinitely variable mixing ratios and a newly designed highly efficient mixer offers performance beyond any existing system. This is important with mixing different viscosity solutions commonly used in protein folding experiments. The SFS-490 is the first stopped-flow designed specifically for stopped-flow CD and the new cell design offers a flat, birefringent free baseline. Seamless control of both the CD spectrometer and stopped-flow system is accomplished from a single window with full kinetic analysis provided as well.

 

 
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