Interest in supercritical fluid chromatography (SFC) applications is growing due to its ability to reduce analysis times, organic solvent consumption and waste production. SFC is well established for small molecule pharmaceutical chiral purifications and is continually expanding into other areas. The exploration of aqueous molecules such as peptides was initially inhibited by beliefs that the successful separation of hydrophilic and polar compounds was unlikely by SFC. However, this application note illustrates the ability of SFC to separate peptides.