Flow Cell Attachment for LCCD LCCD-420
This HPLC cell allows the CD spectrometer to be used as a chiral HPLC detector. A CD chromatogram can be obtained at a fixed wavelength by setting the LCCD-420 Flow cell attachment in the J-815 sample chamber, and then introducing an effluent from an external HPLC. In addition, CD spectrum measurement is possible by stopping HPLC pump flow at each peak top. Optical Rotatory Dispersion
Whereas CD is the differential absorption of right and left handed circularly polarized light, Optical Rotatory Dispersion (ORD) is defined as the rotation of linearly polarized light due to the differential refractive index of circularly polarized light. JASCO offers two choices for measurement of ORD, the ORDE-402
. Linear Dichroism
Linear Dichroism (LD) is defined as the differential absorption of polarized light parallel and perpendicular to an orientation axis. Samples that have an orientation axis will exhibit LD. Recent applications for LD include DNA and bio or synthetic polymers and films. Utilizing a Couette laminar flow cell to orient samples in solution allows measurement of the geometry of a molecule based on the LD signal. Fluorescence Detected Circular Dichroism
FDCD spectropolarimetry is a method of spectrometry to measure the difference in emission intensities that arises when an optically active sample is excited by left and right circularly polarized beams of lights. Emission perpendicular to the excitation light can be measured. The FDCD (Fluorescence detected circular dichroism) is particularly useful when measuring multichromophoric systems such as proteins, because FDCD can selectively measure the CD of a specified fluorescent chromophore.
Simple FDCD measurements are easily obtained using the PDF-425 or MPTC-490 Peltier systems and modulated CD detector. When samples are free from Fluorescence Anisotropy this method is effective due to low photoselection artifact. For samples exhibiting significant Fluorescence Anisotropy the resulting FDCD spectrum may have considerable error due to large photoselection artifacts. The FDCD-465 accessory is specially designed to reduce or eliminate this artifact. Utilizing an ellipsoidal collection mirror, light emitted from the sample is effectively collected from all angles allowing a greatly improved FDCD spectrum.
to request more information.